BC Walpola1* , KKIU Arunakumara2 and Min-Ho Yoon3
1Department of Soil Science, 2Department of Crop Science, Faculty of Agriculture, University of Ru-huna, Mapalana, Kamburupitiya, Sri Lanka.
3Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, 305-764, Korea.
Abstrsact
Aspergillus awamori, a phosphate solubilizing filamentous fungas,
isolated from waste mushroom bed of Agaricus bisporus in South Korea
was immobilized using various immobilized forms such as calcium alginate,
agar, vermiculite and zeolite to assess the phosphate solubilizing potential
in free and immobilized forms. Phosphate solubilization was carried out in
repeated 48 hrs batch fermentation using the fungal strain. Among the four immobilized forms
used, fungi immobilized with agar were shown to release the highest content of soluble
phosphorus into the medium, followed vermiculite and sodium alginate. However phosphate
solubilization of the fungal strain immobilized with zeolite was found to be very low.
Thus, it can be concluded that a culture technique with immo-bilization especially with
agar is more suitable for solubilization of inorganic phosphate by Aspergillus awamori than other forms.
Key words: Aspergillus awamori, calcium alginate, immobilization, vermiculite, Zeolite
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