Effect of Biological Agents and Botanicals in Controlling Root-Knot Nematodes, Meloidogyne SPP., in Nicotiana tabacum

KF Motha1, R Abeysekara2 and NS Kottearachchi1*

1Department of Biotechnology, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka. Makandura
2Ceylon Tobacco Company (PLC), Mawilmada, Kandy

Abstrsact

A study was conducted to investigate the effectiveness of Pseudomonas fluorescens and Trichoderma viride and two botanicals; ground neem seed and tobacco waste dust for the control of Root-Knot-Nematodes (RKN) in Tobacco. Suppression of RKN was examined under field condition by estimating root knots and parasitic nematode counts. Seedling density, fresh weight, dry weight, shoot length and root length of tobacco also were measured to evaluate the growth performance. Results indicated that of all bio-preparations, ground neem seed treatment has suppressed the RKN to the best level expressing least number of root-knots and parasitic nematodes. Tobacco waste treatment found to be the second best followed by the treatment with P. fluorescens. Although T. viride has not controlled the infection, it has significantly improved the seedling density and vegetative growth. Further experiments should be conducted to determine the combined effect of these bio-preparations.

Key words: Biopesticides, Meloidogyne, Neem, Nicotiana tabacum, Pseudomonas fluorescens, Root Knot Nematodes, Trichoderma viride, Tobacco waste

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* - Corresponding Author

Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya, Sri Lanka

Copyright © 2007 by the Faculty of Agriculture, University of Ruhuna

Print ISSN 1391-3646 Online ISSN 2386-1533