| HWL Pushpakumari1* , WLG Samarasinghe2, and SGJN Senanayake1
1Dept. of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Mapalana, Kamburupitiya
2Plant Genetic Resources Centre, Gannoruwa, Peradeniya
Abstrsact
Fourteen Kolikuttu accessions showing morphological variation and Fusarium wilt tolerance were collected. Extracted DNA was subjected to PCR using six SSR primers. PCR products were separated by PAGE and bands were visualized, scored and statistically analyzed using POPGENE 1.32 version to obtain the dendrogram by genetic distance matrix. Out of 19 SSR alleles amplified, 12 were polymorphic resulting in 63.16% loci polymorphism. Based on the dendrogram, three different ‘Kolikuttu’ accessions known as “Athdath Puwalu”, the accession collected from Telijjawila, and accession collected from Angunakolapelassa were clustered separately. These three accessions could be considered as different genotypes.
Key words: DNA Typing, Dessert Banana, Cultivar Kolikuttu
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